Fig. 6

Influence of temperature on the hydrolytic activity and stability of TwCel5 displaying the temperature dependency on the hydrolysis of β-glucan performed in 50 mM sodium phosphate buffer, pH 7.5, using 0.5% (w/v) β-glucan, 0.5 M NaCl and 50 nM enzyme variants, incubated at different temperatures ranging from 5 ºC to 30 ºC for 60 min. The reducing sugars values are mean and standard deviation derived from triplicates