Fig. 2

3-HP production in yeast mitochondria with the optimized expression of MCR genes. A Intact and dissected MCR gene expressed in the cytosol and mitochondria. cMCR_p, the strain harboring pYC01 with intact MCR targeted into the cytosol; mMCR_p, the strain harboring pMCR1 with intact MCR targeted into the mitochondria; mMCR1, the strain with intact MCR integrated at the site XI-3; NC1, the strain with MCR-N and MCR-C integrated at the site XI-3; NmC1, the strain with MCR-N and MCR-mC integrated at the site XI-3; NmC2, the strain with MCR-N and MCR-mC integrated at the sites XI-3 and X-4. B Varied expression of dissected MCR-N and MCR-mC in the mitochondria. NmC2 pUGG1, the strain NmC2 harboring an empty plasmid pUGG1; NmC2 pMCR_N, the strain NmC2 harboring pMCR-N; NmC2 pMCR_mC, the strain NmC2 harboring pMCR-mC; NmC3, the strain with MCR-N and MCR-mC integrated at the sites XI-3, X-4 and Int14. The cultivations were performed in biologically triplicate and error bars represent ± standard errors. Statistical analysis was performed using one-tailed Student’s t test (**p < 0.01)