Fig. 4

Limited flux of TCA cycle impaired cell growth and 3-HP production. A CIT1 downregulation and deletion in strains NmC2 and N3IP. NmC2 pdCas9_CIT, the strain NmC2 harboring pdCas9_CIT; N3IP cit1, the strain N3IP with cit1 deletion. B IDH1/2 downregulation and deletion in NmC2 strains with and without IDP1 and POS5 overexpression. NmC2 pdCas_IDH, the strain NmC2 harboring pdCas_IDH; NmC2 IDP1 idh1, the strain NmC2 IDP1 with idh1 deletion; NmC2 IDP1 idh2, the strain NmC2 IDP1 with idh2 deletion; N2IP idh1, the strain N2IP with idh1 deletion; N2IP idh2, the strain N2IP with idh2 deletion. The cultivations were performed in biologically triplicate and error bars represent ± standard errors