Fig. 1

Boosting the epoxidation of squalene by degrading ERG7. A Schematic representation of the PT synthesis pathway on the basis of the native ergosterol synthesis pathway in yeast and the revealed negative effect of ERG7 on ERG1. tHMG1, 3-hydroxy-3-methylglutaryl-coenzyme-A reductase 1; IDI1, isopentenyl-diphosphate delta-isomerase; ERG20, FPP synthase; ERG9, squalene synthase; ERG1, squalene monooxygenase; ERG7, lanosterol synthase; IPP, isopentenyl pyrophosphate; DMAPP, Dimethylallyl diphosphate; FPP, farnesyl diphosphate; SQO, 2,3-oxidosqualene; SDO, 2,3:22,23-dioxidosqualene; PTs, Polycyclic triterpenoids. B GC chromatograms of ERG7-degraded strains and the control strain S01. C–H Effect of degrading ERG7 by N-degron-mediated protein degradation strategy on the corresponding metabolites of squalene (C), SQO (D), SDO (E), lanosterol (F), ergosterol (G) and OD₆₀₀ (H). Specific peak area is defined as the peak area in the unit dry cell weight. GC–MS analysis of SQO and SDO are provided in Additional file 1:Fig. S2. All values presented are the means of three biological replicates, and error bars represent standard deviations