Fig. 2

Identifying the regulatory effect of ERG7 on ERG1. A The changes in mRNA levels of ERG1 and ERG7 in strain S03 compared to strain S01. The data are obtained by transcriptome sequencing and analyzed using HTSeq (v.0.11.1). When |log2 Fold Change|> 1 and P-Val < 0.05, the mRNA level is determined to be significantly upregulated or downregulated. B The comparison of ERG1 protein stability in ERG7-degraded strain S03 and the control strain S01 by fluorescence labeling analysis. GFP is fused to the N-terminus of ERG1. C The subcellular localization of ERG1 in ERG7-degraded strains and the control strain S01 analyzed by Laser scanning confocal microscopy. GFP is fused to the N-terminus of ERG1 and mCherry is fused to the C-terminus of ERG7. D The comparison of GC chromatograms of Erg7-modified strains S03K7, S03Gg, S03Tf, S03Pb and control strains S01 and S03. E The GC chromatograms of strains S01, S03 and S06. The concentrations of the corresponding metabolites are provided in Additional file 1: Fig. S3. All values presented are the means of three biological replicates, and error bars represent standard deviations