Fig. 4

LaeA is involved in modulating the methylation levels of H3K9. a M. thermophila LaeA protein localized to the nucleus. GFP was fused to the C-terminal end of LaeA. Nuclei were stained with the DNA-specific dye 4, 6-diamidino-2-phenylindole (DAPI). b Global methylation levels of H3K4, H3K9, and H3K27 in histone protein extracts from strain WT and ΔlaeA when grown on glucose for 24 h. Western blot assay of extracted histone of WT and ΔlaeA strains, which were carried out with specific antibodies for histone H3, H3K4me3, and H3K9me3. c Western blot assay of extracted histone protein from strains WT, ΔlaeA::LaeAM1, ΔlaeA::LaeAM2, and ΔlaeA::LaeAM3, with a specific antibody for H3K9me3. d Chromatin immunoprecipitation followed by sequencing with antibodies against H3K9me3 of strains ΔlaeA and WT, after growth on glucose for 24 h. Venn diagram exhibiting amounts of genes which regions showing H3K9 trimethylation peaks (Total 2242 genes; 2050 genes in WT strain and 1212 genes in ΔlaeA,). e GO analysis of genes with differential H3K9me3 levels in ΔlaeA relative to WT as determined by chromatin immunoprecipitation sequencing (ChIP-seq). Down-regulated, representing genes with down-regulated trimethylation level in ΔlaeA; Up-regulated, representing genes with up-regulated trimethylation level in ΔlaeA