Fig. 1

Overview of the reverse β-oxidation pathway and the S. cerevisiae’s metabolic modifications used in this study to produce medium-chain fatty acids. The reverse β-oxidation pathway reactions (red arrows) are depicted with the isoenzymes tested in each reaction during this study. The intermediates and products after one cycle (in blue), two cycles (in orange), three cycles (in green), and four cycles (in grey) run of the reverse β-oxidation pathway are shown. The reactions knocked out in the different strains used in this study are displayed with a red cross. The knocked-out reactions generated during this study (gpd2, tes1, faa2) are highlighted in bold. The reaction of tes1 is shown in blue