Fig. 4

Impact of the β-hydroxyacyl-CoA dehydrogenase selected on the production of MCFA. A Production of butyric acid (light grey) by GDY15 expressing the reverse β-oxidation pathway variants (ACBV007: ^CnBktB, ^CaHbd, ^CaCrt, ^TdTer, GDV151: ^CnBktB, ^YlHad, ^CaCrt, ^TdTer, FHV022: ^CnBktB, ^CnPaaH1, ^CaCrt, ^TdTer) or with the empty vector (EV) after 75 h of fermentation in buffered synthetic medium (SM) without uracil. B Production of hexanoic (white), octanoic (black) and decanoic acid (dark grey) by GDY15 expressing the reverse β-oxidation pathway variants (ACBV007, GDV151, FHV022) or with the empty vector (EV) after 75 h of fermentation in buffered synthetic medium (SM) without uracil. Error bars represent the standard deviation between four independent replicates