Fig. 5

Growth and MCFA production with different enoyl-CoA. A Production of butyric (light grey), hexanoic (white), octanoic (black) and decanoic acid (dark grey) by GDY15 expressing the reverse β-oxidation pathway variants (FHV022: ^CnBktB, ^CnPaaH1, ^CaCrt, ^TdTer), (FHV018: ^CnBktB, ^CnPaaH1, ^CnCrt2, ^TdTer), (GDV144: ^CnBktB, ^CnPaaH1, ^YlECH, ^TdTer) or with the empty vector (EV) B Growth of GDY15 with EV (white square), FHV018 (white circle), FHV022 (white diamond) or GDY144 (white triangle) over 75 h in buffered synthetic medium (SM) without uracil. Error bars represent the standard deviation between three independent replicates