Fig. 8

Effect of increasing butyryl-CoA availability on MCFA production. A Production of butyric (light grey), hexanoic (white), octanoic (black) and decanoic acid (dark grey) in GDY15 expressing the MCFA producing pathway alone (GDV124: ^CnBktB, ^CnPaaH1, ^CnCrt2, ^TdTer), in combination with GDV098 (Erg10, ^CaHbd, ^CaCrt, ^TdTer) or with empty vector (EV_URA + EV_HIS) controls. B Growth of GDY15 with EV (white square), EV_URA + GDV124 (white triangle, or GDV098 + GDV124 (white diamond) over 75 h in synthetic medium (SM) without uracil or histidine. Error bars represent the standard deviation between three independent replicates.