Table 1 List of yeast strains and plasmids used in this study

CENPK2-1c

MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2

EUROSCARF

SHY34

MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2, Δfas1, Δfas2, Δfaa2

[63]

VSY0

MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2 adh1::loxP adh2Δ::LEU2 adh3::loxP adh4Δ::loxP adh5::loxP

[49, 50]

GDY15

MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2 adh1::loxP adh2Δ::LEU2 adh3::loxP adh4Δ::loxP adh5::loxP gpd2Δ

This study

GDY16

MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2 adh1::loxP adh2Δ::LEU2 adh3::loxP adh4Δ::loxP adh5::loxP gpd2Δ tes1Δ

This study

GDY19

MATa; ura3-52; trp1-289; leu2-3_112; his3 Δ1; MAL2-8C; SUC2 adh1::loxP adh2Δ::LEU2 adh3::loxP adh4Δ::loxP adh5::loxP gpd2Δ faa2Δ

This study

GDY27

GDY15 Δura3::pHHF2-^CnbktB-tENO2, pCCW12-^CnpaaH1-tIDP1, pENO2-^Cacrt-tPGK1, pTDH3- ^Tdter -tADH1, KanMX

This study

GDY28

GDY15 Δura3:: pHHF2- ^cnbktB -tENO2, pCCW12-^CnpaaH1-tIDP1, pENO2-^Cncrt2-tPGK1, pTDH3- ^Tdter -tADH1, KanMX

This study

GDY29

GDY15 Δura3:: pHHF2-cnBktB-tENO2, pCCW12-^CnpaaH1-tIDP1, pENO2-^Ylech-tPGK1, pTDH3- ^Tdter -tADH1, KanMX

This study

ACBV007

URA3, CEN6ARS4, Kanamycin R, ColE1, pHHF2- ^cnbktB -tENO2, pCCW12- ^Cahbd -tIDP1, pENO2-^Cacrt-tPGK1, pTDH3- ^Tdter -tADH1

This study

GDV098

URA3, CEN6ARS4, Kanamycin R, ColE1, pPGK1-erg10-tENO2, pCCW12- ^Cahbd -tIDP1, pENO2- ^Cacrt -tPGK1, pTDH3- ^Tdter -tADH1

This study

FHV022

URA3, CEN6ARS4, Kanamycin R, ColE1, pHHF2- ^cnbktB -tENO2, pCCW12- ^CnpaaH1-tIDP1, pENO2-^Cacrt-tPGK1, pTDH3- ^Tdter -tADH1

This study

GDV151

URA3, CEN6ARS4, Kanamycin R, ColE1, pHHF2- ^cnbktB -tENO2, pCCW12- ^Ylhad-tIDP1, pENO2-^Cacrt-tPGK1, pTDH3- ^Tdter -tADH1

This study

FHV018

URA3, CEN6ARS4, Kanamycin R, ColE1, pHHF2- ^cnbktB -tENO2, pCCW12-^CnpaaH1-tIDP1, pENO2-^Cncrt2-tPGK1, pTDH3- ^Tdter -tADH1

This study

GDV144

URA3, CEN6ARS4, Kanamycin R, ColE1, pHHF2- ^cnbktB -tENO2, pCCW12- ^CnpaaH1-tIDP1, pENO2- ^Ylech -tPGK1, pTDH3- ^Tdter -tADH1

This study

GDV116

URA3, CEN6ARS4, Kanamycin R, ColE1, pHHF2- ^cnbktB -tENO2, pCCW12- ^CnpaaH1-tIDP1, pENO2- ^Cncrt2-tPGK1, pTDH3- ^Egter -tADH1

This study

GDV124

HIS3, CEN6ARS4, Kanamycin R, ColE1, pHHF2- ^cnbktB -tENO2, pCCW12- ^CnpaaH1-tIDP1, pENO2- ^Cncrt2-tPGK1, pTDH3- ^Tdter -tADH1

This study

pVS6

Ampicillin R, ColE1, GPD2 5ʹ pHXT7-erg10-tVMA16, pPGK1- ^Cahbd -tEFM1, pTPI1-CaCRT-tYHI9, pPYK1-^Tdter -tIDP1, pADH1-CaADHE2-tRPL3, pTDH3-EcEutE-tRPL41B, KanMX, GPD2 3ʹ

[49]

pVS1

Ampicillin R, ColE1, GPD2 5ʹ pHXT7-erg10-tVMA16, pPGK1-^Cahbd-tEFM1, pTPI1-CaCRT-tYHI9, loxP, KanMX, loxP, pPYK1- ^Egter -tIDP1, pADH1-CaADHE2-tRPL3, GPD2 3ʹ

[49]

pRCCK_GD01

KanMX,2µ, Ampicillin R, ColE1, Cas9, gRNA for GPD2 deletion

This study

FHV016

KanMX,2µ, Ampicillin R, ColE1, Cas9, gRNA for TES1 deletion

This study

pRCCK_SH06

CloNat,2µ, Ampicillin R, ColE1, Cas9, gRNA for FAA2 deletion

Sandra Born (Boles lab)

GDV143

Kanamycin R, ColE1, URA3 5ʹ UTR, pHHF2- ^cnbktB -tENO2, pCCW12- ^CnpaaH1-tIDP1, pENO2- ^Cncrt2-tPGK1, pTDH3- ^Tdter -tADH1, KanMX, URA3 3ʹ UTR

This study

GDV149

Kanamycin R, ColE1, URA3 5ʹ UTR, pHHF2- ^cnbktB -tENO2, pCCW12- ^CnpaaH1-tIDP1, pENO2-^Cacrt-tPGK1, pTDH3- ^Tdter -tADH1, KanMX, URA3 3ʹ UTR

This study

GDV143

Kanamycin R, ColE1, URA3 5' UTR, pHHF2- ^cnbktB -tENO2, pCCW12- ^CnpaaH1-tIDP1, pENO2- ^Cncrt2-tPGK1, pTDH3- ^Tdter -tADH1, KanMX, URA3 3' UTR

This study

GDV150

Kanamycin R, ColE1, URA3 5ʹ UTR, pHHF2- ^cnbktB -tENO2, pCCW12- ^CnpaaH1-tIDP1, pENO2- ^Ylech -tPGK1, pTDH3- ^Tdter -tADH1, KanMX, URA3 3ʹ UTR

This study

LBGV022

URA3, CEN6ARS4, Kanamycin R, ColE1

Leonardo J. Beltran (Boles lab)

LBGV024

HIS3, CEN6ARS4, Kanamycin R, ColE1

Leonardo J. Beltran (Boles lab)