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Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Characterization of Auxenochlorella protothecoides acyltransferases and potential of their protein interactions to promote the enrichment of oleic acid

Fig. 2

Growth, lipid variation, and ApDGATs expression of UTEX 2341 under heterotrophic condition (LM, as a control), LM-N, and LM-Cd. The square solid lines represent LM, the circular dotted lines represent LM-N, and the triangular dotted lines represent LM-Cd culture. The neutral lipids (TAGs) were qualitatively and quantitively analyzed by BODIPY ® 505/515 staining (d) and TLC (e), respectively. Relative TAG content was quantified by Image J software. The relative expression levels of ApDGAT1, ApDGAT2a, and ApDGAT2b in LM, LM-N, and LM-Cd were monitored by quantitative real-time PCR (qPCR) (f, g). Each sample was analyzed in biological and technical triplicates. Each data point represents the average of three biological replicates. The values represent the mean ± SD (n = 3)

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