Fig. 2From: Characterization of Auxenochlorella protothecoides acyltransferases and potential of their protein interactions to promote the enrichment of oleic acidGrowth, lipid variation, and ApDGATs expression of UTEX 2341 under heterotrophic condition (LM, as a control), LM-N, and LM-Cd. The square solid lines represent LM, the circular dotted lines represent LM-N, and the triangular dotted lines represent LM-Cd culture. The neutral lipids (TAGs) were qualitatively and quantitively analyzed by BODIPY ® 505/515 staining (d) and TLC (e), respectively. Relative TAG content was quantified by Image J software. The relative expression levels of ApDGAT1, ApDGAT2a, and ApDGAT2b in LM, LM-N, and LM-Cd were monitored by quantitative real-time PCR (qPCR) (f, g). Each sample was analyzed in biological and technical triplicates. Each data point represents the average of three biological replicates. The values represent the mean ± SD (n = 3)Back to article page