Fig. 3

Functional complementation of acyltransferases in the TAG-deficient yeast strain H1246. a TLC analysis of lipids extracted from H1246 cells transformed with ApDGATs. INVSc1 and H1246 harboring the empty vector (EV) were used as positive and negative controls, respectively. b BODIPY staining of H1246 cells with ApDGATs expressed. Left, fluorescent field; middle, bright field; right, merged field. Green fluorescence indicates the BODIPY-bound lipid droplets. Bars = 7.5 µm. c The growth curve of H1246 transformed with EV, ApDGAT1, and ApDGAT2b. d The fatty acid content of H1246 transformed with EV, ApDGAT1, and ApDGAT2b. e Fatty acid composition of H1246 transformed with EV, ApDGAT1, and ApDGAT2b. Data are mean ± SD of three biological replicates. Different letters indicate significant differences at p < 0.05, as determined by one-way ANOVA with Tukey’s multiple comparisons test