Fig. 2

Effects of pH (a), temperature (b), reaction time (c) or enzyme dosage (d) on the saccharification of CBAX1 or CBAX2 by enzyme blend E_CBAX1. Except as indicated, the hydrolysis reaction was carried out in 200 μL sodium acetate buffer (0.1 M, pH 5.0) containing 4 mg CBAX1 (or CBAX2) and 50 μg (1.25%, g protein/g substrate) crude enzymes at 50 °C for 48 h. E_CBAX1, the enzyme blend produced by P. parvum 4-14 using CBAX1 as carbon source. Error bars represent standard deviations from three repeated measurements