Fig. 3

Analysis of monosaccharide released from CBAX by enzyme blend E_CBAX1. a, b Time-course of liberation of monosaccharide from CBAX1 (a) or CBAX2 (b) during the enzymatic hydrolysis. c, d Monosaccharide liberation from CBAX1 (c) or CBAX2 (d) by the enzyme blend at different dosages (0.12–5.0%, g protein/g substrate). Except as indicated, the hydrolysis reaction was carried out in 200 μL sodium acetate buffer (0.1 M, pH 4.0) containing 4 mg CBAX1 (or CBAX2) and 50 μg (w/w, 1.25%) crude enzymes at 50 °C for 48 h. E_CBAX1, the enzyme blend produced by P. parvum 4-14 using CBAX1 as the sole carbon source. Error bars represent standard deviations from three repeated measurements