Fig. 4

Saccharification of CBAX1 (a) or CBAX2 (b) by the enzyme blends E_CBAX1 and/or CTec3. The enzymatic hydrolyses were carried out in a 2-mL tube containing 0.2 mL sodium acetate buffer (0.1 M, pH 4.0), 4 mg substrate and the corresponding enzymes, at 50 °C and 800 rpm for 3 days. The released monosaccharides were quantified by HPLC analysis. CTec3, commercial T. reesei cellulase Cellic^® CTec3; E_CBAX1, the enzyme blend produced by P. parvum 4-14 using CBAX 1 as carbon source. The data are the averages from three repeated measurements. In the same group, different letters above the error bars indicate significant differences between treatments (LSD test, P < 0.05)