Fig. 5

Saccharification of CBR by the enzyme blends E_CBR, E_CBAX1 and CTec3 separately (a), or the enzyme combinations of E_CBR and CTec3 (b). The enzymatic hydrolyses were carried out in a 2-mL tube containing 1 mL sodium acetate buffer (0.1 M, pH 4.5), 100 mg substrate and the corresponding enzymes, at 50 °C and 1200 rpm for 4 days. The released monosaccharides were quantified by HPLC analysis. CTec3, commercial cellulase Cellic^® CTec3; E_CBAX1 and E_CBR, the enzyme blends produced by P. parvum 4-14 using CBAX1 or CBR as carbon source, respectively. The data are the averages from three repeated measurements. In the same group, different letters above the error bars indicate significant differences between treatments (LSD test, P < 0.05)