Insights into the capability of the lignocellulolytic enzymes of Penicillium parvum 4-14 to saccharify corn bran after alkaline hydrogen peroxide pretreatment

Table 3 Enzymatic activities of crude proteins produced by P. parvum 4-14 using different carbon sources

Enzyme activity (U/mg protein)	E_CBAX1	E_CBAX2	E_CBR
Xylanase	48.03 ± 1.28	52.39 ± 2.09	42.90 ± 1.24
β-Xylosidase	15.03 ± 0.03	14.67 ± 1.30	7.72 ± 0.08
α-Galactosidase	22.60 ± 0.11	21.11 ± 0.77	12.58 ± 1.39
α-Xylosidase	0.21 ± 0.00	0.30 ± 0.04	0.08 ± 0.00
α-l-Arabinofuranosidase	9.63 ± 0.44	9.88 ± 0.60	5.11 ± 0.17
CMCase	2.92 ± 0.04	3.06 ± 0.17	14.17 ± 0.14
Cellulase^a	0.12 ± 0.00	0.09 ± 0.00	0.41 ± 0.00

E_CBAX1, E_CBAX2, and E_CBR, the enzyme blends produced by the fungus using CBAX1, CBAX2, or CBR as the sole carbon source, respectively. All enzyme blends were subjected to salting-out and dialysis treatments before use. The enzymatic reactions were carried out in 0.1 M sodium acetate buffer (pH 5.0) at 45 °C for 10 min, and the activities were determined as described in the “Methods” section.
^aEnzyme activity was measured using PASC as a substrate. The data represent the averages of three independent assays

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