Fig. 8

Effect of transcription factors Hsf1, Msn2 and Msn4 on the concentration of ethyl acetate production. a Ethyl acetate production of mutant strains overexpressing HSF1, MSN2 and MSN4 genes in corn synthesis medium using ISH as parental strains. b Relative transcription levels of HSP30 gene in strains ISH::HSF1, ISH::MSN2 and ISH::MSN4. c Ethyl acetate concentration of strains ISH::HSF1 and ISH::HSP30::HSF1 fermented in YPD-20 medium. d Comparison of four mutant strains with higher ethyl acetate production than the ISP strain. The value represented mean ± SD (n = 3). Statistical significance is denoted as **P < 0.01, *P < 0.05. The lowercase letters a, b, c and d indicate significant differences as determined using Ducan’s multiple range test (P < 0.05)