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Fig. 9 | Biotechnology for Biofuels and Bioproducts

Fig. 9

From: Genome-wide analysis reveals Hsf1 maintains high transcript abundance of target genes controlled by strong constitutive promoter in Saccharomyces cerevisiae

Fig. 9

Overexpression of HSF1 alleviated the repression of the transcription level of target gene ATF1. a ATF1 transcription levels of strains IS45, ISP, ISH, ISH::HSP30 and ISH::HSP30::HSF1 were detected by RT-PCR at 2, 4, 8, 12, 24 h. Sample IS45-2h was used to calculate the relative transcription level of ATF1 gene in other samples. Error bars represent standard deviation among three technical replicates. b Relative transcription levels of HAC1 and IRE1 genes in strain ISH::HSP30::HSF1 compared with that of strain ISH::HSP30. The value represented mean ± SD (n = 3). Statistical significance is denoted as **P < 0.01, *P < 0.05

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