Fig. 9From: Genome-wide analysis reveals Hsf1 maintains high transcript abundance of target genes controlled by strong constitutive promoter in Saccharomyces cerevisiaeOverexpression of HSF1 alleviated the repression of the transcription level of target gene ATF1. a ATF1 transcription levels of strains IS45, ISP, ISH, ISH::HSP30 and ISH::HSP30::HSF1 were detected by RT-PCR at 2, 4, 8, 12, 24 h. Sample IS45-2h was used to calculate the relative transcription level of ATF1 gene in other samples. Error bars represent standard deviation among three technical replicates. b Relative transcription levels of HAC1 and IRE1 genes in strain ISH::HSP30::HSF1 compared with that of strain ISH::HSP30. The value represented mean ± SD (n = 3). Statistical significance is denoted as **P < 0.01, *P < 0.05Back to article page