Fig. 5

Effects of plc-e on cellulase production after Zn²⁺ addition. pNPCase activity (a), CMCase activity (b) of Trichoderma reesei RUT-C30 and Δplc-e cultured for 2, 3, or 4 days in liquid MM with or without 3 mM Zn²⁺ and 1% (w/v) Avicel as the sole carbon source, respectively. The mRNA levels of cbh1 (c), cbh2 (d), egl1 (e), and egl2 (f) were also detected. The final values are presented as the means ± standard deviation (SD) of three independent experimental results. Asterisks indicate significant differences compared with the control (*P < 0.05, according to Student’s t-test). MM, minimal medium