Fig. 7

Effect of zafA on cellulase production after Zn²⁺ addition. pNPCase activity (a), CMCase activity (b) of Trichoderma reesei RUT-C30 and ΔzafA cultured for 2, 3, or 4 days in liquid MM with or without 3 mM Zn²⁺ and 1% (w/v) Avicel as the sole carbon source, respectively. The expression levels of cbh1 (c), cbh2 (d), egl1 (e), and egl2 (f) were also determined. The final values are presented as the means ± standard deviation (SD) of three independent experimental results. Asterisks indicate significant differences compared to the control (*P < 0.05, according to Student’s t-test). MM, minimal medium