Table 1 Bacterial strains and plasmids used in this study

Strains

 ATCC13032

C. glutamicum wild-type, biotin auxotrophic

ATCC^a

 ATCC21420

C. glutamicum, L-phenylalanine producing strain

ATCC^a

 CALE1

2-phenylethanol tolerant strain derived from ATCC 21420

This study

 CALE2

2-phenylethanol tolerant strain derived from ATCC 21420

This study

 CGPE1

CALE1 (pEC-aro10yjgB)

This study

 CGPE2

CALE1 (pEC-pmkdcyjgB)

This study

 CGPE3

CALE1 (pEC-ipdcyjgB)

This study

 CGPE4

CALE1 (pEC-aro10yahK)

This study

 CGPE5

CALE1 (pEC-pmkdcyahK)

This study

 CGPE6

CALE1 (pEC-ipdcyahK)

This study

 CGPE7

CALE1 (pEC-aro10adhA)

This study

 CGPE8

CALE1 (pEC-pmkdcadhA)

This study

 CGPE9

CALE1 (pEC-ipdcadhA)

This study

 CGPE10

CALE1 (pEC-aro10yahK-aroG^fbrpheA^fbr)

This study

 CGPE11

CALE1 (pEC-aro10yahK-aroHpheA^fbr)

This study

 CGPE12

CALE1 (pEC-aro10yahK-aroG^fbrpheA^fbraroL)

This study

 CGPE13

CALE1 (pEC-aro10yahK-aroG^fbrpheA^fbraroA)

This study

 CGPE14

CALE1 (pEC-aro10yahK-aroG^fbrpheA^fbraroA, pX-ppsAtkt)

This study

 CGPE15

CALE1 (pEC-aro10yahK-aroG^fbrpheA^fbraroA, pX-P_homxylE-ppsAtkt-xylAB)

This study

Plasmids

This study

pEC-xk99E

Kan^R; C. glutamicum / E. coli shuttle vector (P_trc, lacI^q; pGA1, OriV_C.g., OriV_E.c.)

[27]

pXMJ19

Cm^R; C. glutamicum / E. coli shuttle vector (P_tac, lacI^q; pBL1, OriV_C.g., OriV_E.c.);

[27]

pEC-aro10yahK

For overexpression of aro10 and yahK

This study

pEC-aro10yjgB

For overexpression of aro10 and yjgB

This study

pEC-aro10adhA

For overexpression of aro10 and adhA

This study

pEC-pmkdcyahK

For overexpression of pmkdc and yahK

This study

pEC-pmkdcyjgB

For overexpression of pmkdc and yjgB

This study

pEC-pmkdcadhA

For overexpression of pmkdc and adhA

This study

pEC-ipdcyahK

For overexpression of ipdc and yahK

This study

pEC-ipdcyjgB

For overexpression of ipdc and yjgB

This study

pEC-ipdcyahK

For overexpression of ipdc and adhA

This study

pEC-aro10yahK-aroG^fbrpheA^fbr

For overexpression of aro10, yahK, aroG^fbr and pheA^fbr

This study

pEC-aro10yahK-aroHpheA^fbr

For overexpression of aro10, yahK, aroH and pheA^fbr

This study

pEC-aro10yahK-aroHpheA^fbraroL

For overexpression of aro10, yahK, aroG^fbr, pheA^fbr and aroL

This study

pEC-aro10yahK-aroHpheA^fbraroA

For overexpression of aro10, yahK, aroG^fbr, pheA^fbr and aroA

This study

pX-ppsAtkt

For overexpression of ppsA and tkt

This study

pX-P_homxylE-ppsAtkt-xylAB

For overexpression of ppsA, tkt, xylE and xylAB

This study