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Fig. 4 | Biotechnology for Biofuels and Bioproducts

Fig. 4

From: Quantification and mitigation of byproduct formation by low-glycerol-producing Saccharomyces cerevisiae strains containing Calvin-cycle enzymes

Fig. 4

Growth, glucose consumption, ethanol formation, acetaldehyde formation and glycerol formation in anaerobic bioreactor batch cultures of S. cerevisiae strains IME324 (reference strain lacking PRK/RuBisCO bypass) (A), IMX1489 (Δgpd2, non-ox PPP↑, pDAN1-prk, 15 × cbbm, GroES/GroEL)(B), IMX2736 (Δgpd2, non-ox PPP↑, pDAN1-prk, 2 × cbbm, GroES/GroEL)(C), IMX2701 (Δgpd2, non-ox PPP↑, pDAN1-prk-CLN2PEST, 2 × cbbm, GroES/GroEL)(D), IMX2593 (Δgpd2, non-ox PPP↑, pDAN1-prk-19aa, 2 × cbbm, GroES/GroEL)(E) and IMX2608 (Δgpd2, non-ox PPP↑, pANB1-prk, 2 × cbbm, GroES/GroEL) (F) Cultures were grown anaerobically at pH 5 and at 30 °C on synthetic medium containing 20 g L−1 glucose. Representative cultures of independent duplicate experiments are shown, corresponding replicate of each culture shown in Additional file 7: Figure S3

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