Table 4 Plasmids used in this study

p426-GPD

2 µm ori, URA3, pTDH3 -tCYC1 (empty vector)

[63]

pROS10

2 µm ori, KIURA3, gRNA.CAN1-gRNA.ADE2

[61]

pUDR103

2 µm ori, KIURA3, pSNR52-gRNA.SGA1-tSUP4

[13]

pUDR760

2 µm ori, KIURA3, pSNR52-gRNA.prk-tSUP4

This study

pUDE046

2 µm ori, TRP1, pGAL1-prk-tCYC1

[12]

pUDE672

2 µm ori, URA3, pTDH3-eGFP-tCYC1

This study

pUDE1119

2 µm ori, URA3, pTDH3-eGFP19aa-tCYC1

This study

pUDE1120

2 µm ori, URA3, pTDH3-eGFP-CLN2_PEST-tCYC1

This study