Table 5 S. cerevisiae strains used in this study

CEN.PK113-7D

MATa URA3

–

CEN.PK113-5D

MATa ura3-52

–

IMX581

MATa ura3-52 can1::cas9-natNT2

CEN.PK113-5D

[61]

IMX1489

MATa ura3-52 can1::cas9-natNT2 gpd2::non-ox PPP↑ sga1::pDAN1-prk, cbbm (15 copies), groES, groEL pUDR103 (KlURA3)

IMX581

[13, 23]

IMX2288

MATa ura3-52 can1::cas9-natNT2 gpd2::non-ox PPP↑ sga1::pDAN1-prk, cbbm (2 copies), groES, groEL

IMX1489

This study

IMX2545

MATa ura3-52 can1::cas9-natNT2 gpd2::non-ox PPP↑ sga1::sga1-gRNA site, cbbm (2 copies), groES, groEL

IMX2288

This study

IMX2593

MATa ura3-52 can1::cas9-natNT2 gpd2::non-ox PPP↑ sga1::pDAN1-prk-19aa, cbbm (2 copies), groES, groEL pUDR103 (KlURA3)

IMX2545

This study

IMX2608

MATa ura3-52 can1::cas9-natNT2 gpd2::non-ox PPP↑ sga1::pANB1-prk, cbbm (2 copies), groES, groEL pUDR103 (KlURA3)

IMX2545

This study

IMX2701

MATa ura3-52 can1::cas9-natNT2 gpd2::non-ox PPP↑ sga1::pDAN1-prk-CLN2_PEST, cbbm (2 copies), groES, groEL pUDR103 (KlURA3)

IMX2545

This study

IMX2736

MATa ura3-52 can1::cas9-natNT2 gpd2::non-ox PPP↑ sga1::pDAN1-prk, cbbm (2 copies), groES, groEL pUDR103 (KlURA3)

IMX2545

This study

IME678

MATa ura3-52 can1::cas9-natNT2, pUDE672 (URA3)

IMX581

This study

IME681

MATa ura3-52 can1::cas9-natNT2, pUDE1119 (URA3)

IMX581

This study

IME682

MATa ura3-52 can1::cas9-natNT2, pUDE1120 (URA3)

IMX581

This study