Table 1 Operating conditions of the bioreactor
Phase | Period (days) | ELR (g L−1 d−1) [mM d−1] | HRT (h) | Ethanol inflow (mM) | Remark | ||
|---|---|---|---|---|---|---|---|
Batch | 0 | – | 2.1 | – | – | – | – |
A | 2.1 | – | 3 | 1.7 [35] | 36 | 51 | Start of the continuous operation |
B | 3 | – | 17.8 | 1.7 [35] | 36 | 51 | Gas flowrate increased to 0.07 vvm |
C | 17.8 | – | 29.7 | 3.1 [64] | 36 | 95 | – |
D | 29.7 | – | 32.9 | 8.0 [171] | 42 | 300 | – |
E.1 | 32.9 | – | 43.7 | 6.3 [122] | 54 | 300 | – |
E.2 | 43.7 | – | 100.7 | 6.3 [122] | 54 | 300 | Technical issuesa |
F | 100.7 | – | 155.7 | 6.7 [145] | 54 | 319 | Technical issues fixed and new medium tank |
G | 155.7 | – | 160 | 6.7 [145] | 54 | 320 | pH increased to 7.3 |