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Fig. 3 | Biotechnology for Biofuels and Bioproducts

Fig. 3

From: Transcriptomic and metabolomic analyses revealed regulation mechanism of mixotrophic Cylindrotheca sp. glycerol utilization and biomass promotion

Fig. 3

Light regulation on glycerol utilization in Cylindrotheca sp. a Proposed glycerol utilization pathway in microalgae; TCA: tricarboxylic acid; b changes in the transcriptional level of DEGs encoding enzymes in glycerol utilization pathway are displayed in a heatmap. GK: glycerol kinase; GPDH: glycerol-3-phosphate dehydrogenase [NAD (+)]; TIM: triosephosphate isomerase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; PGK: phosphoglycerate kinase; GAPN: glyceraldehyde-3-phosphate dehydrogenase (NADP+); PGAM: phosphoglycerate mutase; ENO: enolase; PK: pyruvate kinase; PDHA/PDHB: pyruvate dehydrogenase E1 component alpha/beta subunit; DLAT: dihydrolipoamide acetyltransferase; AUTO: autotrophy; MIXO: mixotrophy; c principal component analysis (PCA) for the genes involved in glycerol metabolism; d fold changes in gene expressions at point B and C, quantified by FPKM, compared to those at point A in each trophic mode. Analysis was carried out for biological triplicates and results are displayed as mean ± SD, n = 3

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