Fig. 4

Bioinformatics analysis of the RSR1 gene. A The Pfam database was used to predict the functional domain of RSR1(TRE76880). A catalytic domain at position 11–177 (http://pfam.xfam.org) was identified. B Phylogenetic analyses of RSR1 protein and its orthologs. BLASTP of NCBI was used to find representative RSR1 orthologs. Then, amino acid sequence alignment and phylogenetic analysis was performed with ClustalW and the maximum likelihood tree was generated using MEGA7. The bootstrap supports calculated per 1,000 bootstrap replicates are represented by numbers on the tree branches