Fig. 1

Activity of the full-length Arabidopsis WRI1 (WRI1), and a truncated form (ΔWRI1) in tobacco leaves. a Semi-quantitative reverse-transcription PCR analysis of individual leaves after infiltration with p35S:WRI1 or p35S:ΔWRI1 strains. Numbers in parentheses indicate biological replicates. b Thin-layer chromatography analysis of triacylglycerols (TAG) in the infiltrated leaves. The position of TAG and the lipid reference triheptadecanoin is indicated by an arrow. c Quantification of total TAG levels in the infiltrated leaves using gas chromatography analysis. FW, fresh weight. Mean value ± SD from three biological replicates. Asterisks indicate a statistical difference compared to untreated control leaves, significant at *p ≤ 0.05 or **p ≤ 0.01 (Student´s t-test)