Fig. 4

TAG levels in reproductive tissues of wild-type Indica rice Minghui 86 (MH86), and derived AtWRI1 transformants. a Relative expression of WRI1 and ∆WRI1 transgenes in the seed coat (SC), endosperm (EN) and embryo (EM). b Relative expression of genes in fatty acid synthesis, as analyzed in the seed coat, endosperm, and embryo. Mean value ± SD from three biological replicates for each genotype. Asterisks indicate a statistical difference compared to the wild type, significant at *p ≤ 0.05 or **p ≤ 0.01 (Student´s t-test). c Western blot analysis of OsPK-cyto and OsACC protein levels in leaf and stem. Quantifications of signal intensities are shown in Additional file 1: Table S1. daf: day after fertilization; WRI1: full-length AtWRI1; ΔWRI1: truncated AtWRI1