Fig. 5

Fatty acid and starch levels in reproductive tissues of wild-type Indica rice Minghui 86 (MH86) plants, and derived AtWRI1 transformants. a Quantification by gas chromatography analysis of TAG level in endosperm, and in mature seeds without embryo. b TAG level in whole seeds at different times after fertilization (daf). c Transmission electron microscopy analysis of endosperm structures in MH86 and a transgenic line (ΔWRI1). S, starch; P, proteins; O, oil body. Scale bars (5 µm or 2 µm) are indicated. d Starch level in mature seeds without embryo. Mean value ± SD from three biological replicates for each genotype. FW, fresh weight; DW, dry weight; daf: day after fertilization; WRI1: full-length AtWRI1; ΔWRI1: truncated AtWRI1. Asterisks indicate a statistical difference compared to wild-type control plants, significant at *p ≤ 0.05 or **p ≤ 0.01 (Student´s t-test)