Fig. 2

Structure and sequence features of AATs. a Structure diagram of FvAAT (AAT from Fragaria vesca [119]). 3D homology modeling of FvAAT was conducted using RoseTTAFold with 2BGH (PDB accession code) as the template. FvAAT consists of two domains (yellow and orange), joined by a crossover loop (magenta). Benzyl alcohol and acetyl-CoA (shown as sticks) were chosen as the representative substrates for molecular docking with FvAAT. The overall fold is represented by a cartoon and ribbon diagram. All structural figures in this article were prepared using Pymol software. b Sequence conservation analysis diagram of AATs using Weblogo (http://weblogo.berkeley.edu/logo.cgi). c Multiple sequence alignments of AATs. Comparison of amino acid sequences was aligned using the ClustalW2 server. The residues marked with red asterisk are the important amino acids identified. The important motifs in the black dotted box and a loop connecting two domains are in the red dotted box. FvAAT (NP_001295454); SAAT (Strawberry, AF193789_1); RhAAT (Rosa hybrid cultivar, AAW31948); FcAAT (Fragaria chiloensis, ACT82247); VpAAT (ACT82248); PpAAT (Prunus persica, XP_007209131); MdAAT (Malus domestica, AAT2_MALDO); BanAAT (Musa acuminata, CAG1859434); CmAAT1 (KAA0066373); CmAAT2 (AF468022_1); CmAAT3 (NP_001315395); CmAAT4 (NP_001315389). FvAAT has 463 amino acids with a molecular weight of 51.4 kDa, which has two conserved regions typical of the BADH superfamily HXXXD and DFGWG (amino acids 381–385 in FvAAT) near the C-terminus [60]