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Table 1 Characteristics of high-moisture Italian ryegrass before and during ensiling

From: The metabolome and bacterial composition of high-moisture Italian ryegrass silage inoculated with lactic acid bacteria during ensiling

  

pH

LA

AA

PA

BA

DM

WSC

ADF

NDF

   

g/kg DM

g/kg DM

g/kg DM

g/kg DM

g/kg

g/kg DM

g/kg DM

g/kg DM

Fresh forage

6.73

–

–

–

–

177.41

176.63

53.89

181.33

Silage

          

Day

Treatments

        

3

CK

6.66a

11.35Ca

0.39c

0.04Bb

1.25

195.94ab

71.05

94.40

201.83

 

HE

6.69a

16.86Ab

0.45b

1.25A

0.06

205.41a

80.62a

96.95

186.26

 

HO

6.66a

13.26BCb

0.50b

1.43Ab

ND

204.33a

96.71

105.94

184.65

 

M

6.57a

15.24ABb

0.39c

1.49A

ND

198.49

79.06

95.77

186.79

15

CK

6.18Ab

5.49Db

1.20BCb

0.05b

0.38A

205.04Aa

67.65A

90.22

163.00B

 

HE

4.12Bc

10.70Bc

1.59Aa

ND

0.15C

179.44Bb

34.42Bb

105.14

189.61B

 

HO

3.82Dc

7.98Cc

0.87Cb

0.05c

0.24B

195.37AB

70.05A

128.61

231.07A

 

M

3.99Cc

14.47Ab

1.27ABb

ND

0.16C

189.43ABa

46.40B

113.83

205.76AB

60

CK

5.05Ac

6.30Cb

1.77Ba

1.09Ca

ND

189.08Ab

45.31

85.41

163.87

 

HE

5.07Ab

21.35Aa

1.79Ba

3.56A

ND

154.09Bc

27.12b

119.64

185.65

 

HO

4.53Bb

18.53Ba

2.62Aa

2.48Ba

ND

197.08A

46.05

105.34

188.23

 

M

4.93Ab

17.32Ba

2.09Ba

3.29A

ND

165.10Bb

31.29

80.30

162.76

SEM

 

0.06

0.57

0.102

0.101

0.034

4.931

11.858

12.283

20.778

Levels of significance

 Treatments(T)

***

***

*

***

***

***

0.102

0.118

0.533

 Day (D)

 

***

***

***

***

***

***

***

0.328

0.323

 T × D

 

***

***

***

***

***

**

0.699

0.473

0.537

  1. LA lactic acid, AA acetic acid, PA propionic acid, BA butyric acid, DM dry matter, WSC water-soluble carbohydrates, ADF acid detergent fiber, NDF neutral detergent fiber, CK control, HE Lactobacillus buchneri, HO Lactobacillus rhamnosus, M combination of HE and HO, SEM error of the means, ND not detected, T treatment, D ensiling time, T × D interaction of T and D; *P < 0.05; **0.001 < P < 0.01; ***P < 0.001. Values with different uppercase letters (A–C) show significant differences among additives in the same ensiling day; values with different lowercase letters (a–c) show significant differences among ensiling days in the same additive (P < 0.05).