Fig. 1

Whole-cell bioconversion of PCA into PDC. A Scheme of bioconversion of PCA into PDC catalyzed by LigAB and LigC. Time courses of PCA (B) and PDC (C) concentrations catalyzed by LigABC biocatalyst in different reaction buffers. The reactions were conducted in 100 mL shake flasks with 10 mL of 100 mM sodium phosphate buffer (PB, pH 7.0) or 100 mM Tris–HCl (pH 7.0) or M9 medium (pH 7.0), 5 OD₆₀₀ of whole-cell biocatalyst, 5 g/L PCA and incubated at 37 °C and 250 rpm. All data and standard errors were derived from three independent biological replicates