Fig. 4

Effects of temperature and pH on whole-cell bioconversion of PCA into PDC. Time courses of PCA (A) and PDC (B) concentrations catalyzed by 14ABC biocatalyst at different temperatures. The reactions were conducted in 100 mL shake flasks with 10 mL M9 medium, 5 OD₆₀₀ of whole-cell biocatalyst, 5 g/L PCA and incubated at 250 rpm. C Time courses of PDC production catalyzed by 14ABC biocatalyst in the pH range of 5.5 to 7.0. The reactions were conducted in 5-L bioreactors at 37 °C with 1 L M9 medium and 30 OD₆₀₀ of whole-cell biocatalyst. Approximately 10 g/L PCA was supplemented into the bioreactor at one time when the DO level rose. All data and standard errors were derived from three independent biological replicates