Fig. 5

Whole-cell bioconversion of PCA into PDC on a large scale. Time courses of the PDC production catalyzed by the biocatalysts of 2ABC (A), 14ABC (B) and LigABC (C). The reactions were conducted in 5-L bioreactors with 1 L M9 medium and 30 OD₆₀₀ of whole-cell biocatalysts at 37 °C and pH 6.5. Approximately 10 g/L PCA was supplemented into the bioreactor at one time when the DO level rose (indicated with black arrows). The error bars represent the standard deviation of the three replicates, and the DO curve is the result of one run