Fig. 6

Whole-cell bioconversion of DHS into PCA. A Bioconversion of DHS into PCA catalyzed by 3 OD₆₀₀ of biocatalysts expressing different 3-dehydroshikimate dehydratases. E. coli BL21(DE3) strain transformed with pET-30a plasmid was used as the negative control (CK). B Time courses of the conversion ratio catalyzed by 0.2 OD₆₀₀ of AbQuiC biocatalyst in the pH range of 5.5 to 8.0. C Time courses of the conversion ratio catalyzed by AbQuiC biocatalyst with different loads at pH 7.0. The reactions were conducted in 100 mL shake flasks with 60 g/L of DHS and incubated at 37 °C and 250 rpm. All data and standard errors were derived from three independent biological replicates