Fig. 7

Multi-step biosynthesis of PDC from glucose. A Scheme of the multi-step biosynthesis of PDC from glucose. EMP Embden–Meyerhof–Parnas pathway, PPP pentose phosphate pathway, PEP phosphoenolpyruvate, E4P d-erythrose 4-phosphate, DAHP 3-deoxy-d-arabino-heptulosonate-7-phosphate. B Biosynthesis of DHS from glucose by the WJ060 strain through fed-batch fermentation. C Bioconversion of fermentative DHS into PCA catalyzed by 2 OD₆₀₀ of AbQuiC biocatalyst. The reaction was conducted in a 5-L bioreactor at 37 °C and pH 7.0. D Bioconversion of the PCA broth into PDC catalyzed by 2ABC (PsLigABC) biocatalyst. The reaction was conducted in a 5-L bioreactor with ~ 30 OD₆₀₀ of whole-cell biocatalyst at 37 °C and pH 6.5. Approximately 15 g/L PCA was supplemented into the bioreactor at one time when the DO level rose (indicated with black arrows). The error bars represent the standard deviation of the three replicates, and the DO curve is the result of one run