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Table 1 Production of PDC by engineered strains

From: Multi-step biosynthesis of the biodegradable polyester monomer 2-pyrone-4,6-dicarboxylic acid from glucose

Strain

Substrate

Cultivation

Titer (g/L)

Yield

Time (h)

References

P. putida PpY1100 with pDVABC

PCA

5-L bioreactor; fed-batch

~ 11

–

36

[22]

E. coli BL21(DE3) with pRSF-2ABC

PCA

5-L bioreactor; fed-batch

53.46

0.952 mol/mol

22 (15 + 7)

This study

P. putida KT2440 ΔpcaGHΔcrc with pSEVA631-ligABC

p-Coumarate

5-L bioreactor; fed-batch

22.7

1.0 mol/mol

110

[16]

N. aromaticivorans DSM12444 ΔligIΔdesCD

Vanillin and vanillate

0.25-L bioreactor; fed-batch

4.9

0.103 mol/mol

48

[20]

P. putida KT2440 ΔpcaHG::Ptac:ligABC (CJ251)

4-Hydroxybenzoate

2.5-L bioreactor; fed-batch

58

0.81 mol/mol

288

[19]

P. putida PpY1100 with pVapoligVABC

Vanillin and vanillate derived from lignin

Bioreactor; fed-batch

9.21 (50 mM)

–

18

[23]

P. putida PpY1100-dHG with pJFVV2AB and pDVZ21X

Bamboo extract

1-L bioreactor; fed-batch

8.7

0.936 mol/mol

24

[42]

P. putida KT2440 KT2440 ΔpcaHG::Ptac:ligABC ΔvanAB::Ptac:vanABHR199

Equimolar mixture of syringate, p-coumarate, and ferulate

Shake flask

0.79 (4.3 mM)

0.93 mol/mol

24

[21]

E. coli PCA strain and E. coli PDCPCA strain

Terephthalic acid derived from PET waste

Shake flask

0.57 (3.11 mM)

0.99 mol/mol

6

[24]

E. coli BL21(DE3) harboring three plasmids (pACYC-aroFfbr-aroB, pCDF-ubiC-pobA, and pFT-ligABC-qutC)

Algae hydrolysate and glucose

Shake flask

1.22

0.161

24

[25]

E. coli GYT1 with pTacFABC and pBBR1Gfbr-EcA

Glucose

6.6-L bioreactor; fed-batch

16.72

0.201 g/g

96

[27]

E. coli WJ060, E. coli BL21(DE3) with pET30a-AbquiC and E. coli BL21(DE3) with pRSF-2ABC

Glucose

5-L bioreactor; fed-batch

49.19

0.272 mol/mol

66

This study