Fig. 8

(A) Potential activating/ inhibiting compounds added to the assays to determine which conditions PfkB prefer as well as any possible feedback effect from other metabolic sugars. The velocity of the enzyme in each reaction was recorded in the presence of 2.24 mM substrate, 2.24 mM phosphate donor and 2.24 mM effector excluding MgCl₂ (5.6 mM). (B) Relative activity of the enzyme with potential activating/inhibiting compounds added to the assays, compared to assays without any potential effectors other than MgCl₂(5 mM) with ATP as the phosphate donor while (C) utilized GTP. The concentration of PP_i used in the assay was 0.4 mM, while the other effectors remained at 2.24 mM (B, C)