Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles

Table 1 Kinetic characterization of recombinant Cthe_1261(PfkA)

\(V_{\mathrm{max}}(\frac{\mathrm{\mu mol}}{\mathrm{mg min}})\)	K_m (mM)	Phosphate donor^a	Substrate^b
430.5 ± 105.4	2.5 ± 0.96	ATP^c	Fructose-6-phosphate
281.6 ± 8.9	0.2 ± 0.02	GTP^c	Fructose-6-phosphate
169.1 ± 13.8	0.6 ± 0.1	ATP	Fructose-6-phosphate^d
563.9 ± 42.4	1.9 ± 0.2	GTP	Fructose-6-phosphate^d

^aPhosphate donor used in the reactions
^bSubstrate used in the reactions
^cThe concentration of the phosphate donor was varied to determine the K_m of said phosphate donor, while the substrate concentration was held constant. Phosphate donors were used to initiate the reaction, indicated in bold
^dThe concentration of the substrate was varied to determine the K_m, while the concentration of the phosphate donor was held constant at 2 mM. The assay for each concentration whether phosphate donor or substrate was done in triplicate. F6P was used to initiate the reaction, indicated in bold

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