Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles

Table 2 Kinetic characterization of recombinant Cthe_0347(Pfp)

Enzyme	Substrate/phosphate donor	\(V_{\mathrm{max}}(\frac{\mathrm{\mu mol}}{\mathrm{mg min}})\)	K_m (mM)
Cthe_0347	Fructose-6-P/PP_i^a	39.7	0.23^b
	Sedoheptulose-7-P/PP_i	6.294 ± 3.723	3.236^e
	Fructose-6-P^c/ PP_i	100.222 ± 11.6	0.075^d

^aThis assay was done at 50 °C by [37] in comparison to ^c done in this study. ^bK_m of pyrophosphate. ^dK_m of fructose-6-P, ^eK_m of sedoheptulose-7-phosphate while PPi-pyrophosphate (PP_i) concentration was held constant

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