Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles

Table 3 Kinetic characterization of recombinant Cthe_0389(PfkB)

\(V_{\mathrm{max}}(\frac{\mathrm{\mu mol}}{\mathrm{mg min}})\)	K_m (mM)	Phosphate donor^a	Substrate^b
110.5 ± 12.1	0.4 ± 0.1	ATP^c	Fructose
123.9 ± 14.3	0.2 ± 0.08	GTP^c	Fructose
278.4 ± 57.5	1.8 ± 0.6	ATP	Fructose^d
217.5 ± 20.4	0.7 ± 0.2	GTP	Fructose^d

^aPhosphate donor used in the reactions
^bSubstrate used in the reactions
^cThe concentration of the phosphate donor was varied to determine the K_m of said phosphate donor, while the concentration of the substrate was held constant at 2 mM. Phosphate donors were used to initiate the reaction, indicated in bold
^dThe concentration of the substrate was varied to determine the K_m, while the concentration of the phosphate donor was held constant at 2 mM. The assay for each concentration whether phosphate donor or substrate was done in triplicate. F6P was used to initiate the reaction, indicated in bold

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