Fig. 8From: Ethanol tolerance in engineered strains of Clostridium thermocellumCharacterization of a purified AdhE protein. The C. thermocellum adhE gene, with or without the D844Y mutation, was cloned, expressed, and purified in E. coli using a his-tag. The resulting protein was assayed for ALDH and ADH activity with NADH as the cofactor. Error bars represent one standard deviation n ≥ 3Back to article page