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Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Biochemical and structural characterization of a glucan synthase GFGLS2 from edible fungus Grifola frondosa to synthesize β-1, 3-glucan

Fig. 2

Plasmid construction process (A, B). The plasmid pET-30a ( +)-gfgls2classI/III was constructed by inserting the gfgls2classI/III gene into the NdeI and HindIII sites of plasmid pET-30a ( +). C The electrophoretogram of recombinant plasmids and the recombinant plasmid double-digestion product. Lane M: DNA 10,000 maker; lane 1: recombinant plasmid; and lane 2: recombinant plasmid double-digested by NdeI and HindIII. D Affinity purification of rGFGLS2-classI and rGFGLS2-classIII on a Ni–NTA-Sefinose column. Peak I represents the specific activity of miscellaneous proteins and peak II the imidazole-eluted target protein rGFGLS2-classI and rGFGLS2-classIII. E SDS-PAGE of purified pET-30a ( +)-gfgls2classI/III. Lane M: protein marker; lane 1 and 2: affinity-purified crude protein of pET-30a ( +)-gfgls2classI and pET-30a ( +)-gfgls2classIII loaded on the Ni–NTA-Sefinose column; and lane 3 and 4: purified pET-30a ( +)-gfgls2classI and pET-30a ( +)-gfgls2classIII

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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