Table 1 Primers used for cloning gDNA sequences of GFGLS2, and CDS sequences of gfgls2classI and gfgls2classIII

GFGLS2-gDNA-F

ATGGCTTCCAATCCGGGCTATG

cloning of gDNA sequence of GFGLS2 from G. frondosa genome

GFGLS2-gDNA-R

TTAGATATTTTGGCAAACGGT

gfgls2classI-

F-NdeI

GGAATTCCATATGATGGCTTCAAATCCAGGTTA

Cloning the CDS sequence

of gfgls2classI to

pET-30a( +)

gfgls2classI-

R-HindIII

CCCAAGCTTAGCTGAACGCTTTTCAAAAA

gfgls2classIII-

F-NdeI

GGAATTCCATATGTGGAAAGAGGTTTATACGCG

Cloning the CDS sequence

of gfgls2classIII to

pET-30a( +)

gfgls2classIII-

R-HindIII

CCCAAGCTTTATGAAAACCGGGATGACCA