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Table 6 Production of PBPs

From: Phycobiliproteins from microalgae: research progress in sustainable production and extraction processes

PBPs

Microalgae species

Cell disruption + crude extraction

Purification

Purity

Yield (%)

References

PC

Spirulina (Arthrospira) platensis

High pressure homogenization

ATPS + anion-exchange chromatography

6.69

 

[84]

PC

Spirulina (Arthrospira) platensis

Homogenized

Aqueous two-phase extraction

4.32

79

[85]

PC

Spirulina (Arthrospira) platensis

Freezing and thawing + ammonium sulfate fractionation

Anion-exchange chromatography

4.5

14

[86]

PC

Limnotrhrix sp.

Freezing and thawing + ammonium sulfate fractionation

Activated charcoal and chitosan + tangential cross-flow filtration

4.3

8

[87]

PC

Phormidium fragile

Grinding under liquid nitrogen + ammonium sulfate fractionation

Hydrophobic interaction chromatography

4.52

 

[88]

PC

Arthronema

Freeze–thawed

Rivanol treatment

4.52

55

[89]

APC

2.41

35

PC

Spirulina fusiformi

Freezing and thawing

Rivanol treatment

4.3

46

[90]

PC

Aphanizomenon flos-aquae

Ammonium sulfate fractionation

Hydroxyapatite chromatography

4.78

 

[91]

PC

Spirulina (Arthrospira) platensis

Homogenized

Anion-exchange chromatography

3.43

77.3

[92]

PC

Synechocystis aquatilis

Osmotic shock

Expanded bed chromatography + anion-exchange chromatography

 > 4.0

 

[93]

PC

Calothrix sp.

EDTA and lysozyme treatment

Anion chromatography + hydrophobic interaction chromatography

3.5

 

[94]

PC

Euhalothece sp.

Freezing and thawing + ammonium sulfate fractionation

Activated carbon adsorption + UF

5

60

[95]

PC

Anabaena fertilissima UPCCC

Freezing and thawing + ammonium sulfate fractionation

Ion exchange chromatography + size exclusion chromatography

3.28

28

[96]

PC

Synechococcu sp.

Ultrasound + ammonium sulfate fractionation

Ion-exchange (DEAE-cellulose) chromatography

4.03

 

[97]

PC

Spirulina (Arthrospira) platensis

Ultrasound + ammonium sulfate fractionation

Gel-filtration column

3.5

 

[98]

PC

Arthrospira platensis

Osmotic shock

Activated charcoal + Sephadex G 100 + DEAE sepharose fast Flow

3.25

48.2

[99]

PC

Arthrospira platensis

Ultrasound

Activated carbon adsorption

1.23

80

[100]

PC

Spirulina (Arthrospira) platensis

Milling + ammonium sulfate fractionation

Ion exchange chromatography

4.0

 

[101]

PC

Arthrospira platensis

Microwave

Ionic liquids

1.22

 

[102]

PE

0.71

APC

1.03

PC

Spirulina (Arthrospira) platensis

Freezing and thawing

ATPS

2.38

 

[103]

PC

Spirulina (Arthrospira) platensis

Freezing and thawing

ATPS + UF

2.11

 

[104]

C-PC

Spirulina (Arthrospira) platensis

Ultrasound

Liquid biphasic flotation technique

3.49

90.4

[105]

PC

Arthrospira platensis

Ultrasound

ATPS + dialysis + precipitation

4.22

 

[106]