PBPs | Microalgae species | Cell disruption + crude extraction | Purification | Purity | Yield (%) | References |
---|---|---|---|---|---|---|
PC | Spirulina (Arthrospira) platensis | High pressure homogenization | ATPS + anion-exchange chromatography | 6.69 | [84] | |
PC | Spirulina (Arthrospira) platensis | Homogenized | Aqueous two-phase extraction | 4.32 | 79 | [85] |
PC | Spirulina (Arthrospira) platensis | Freezing and thawing + ammonium sulfate fractionation | Anion-exchange chromatography | 4.5 | 14 | [86] |
PC | Limnotrhrix sp. | Freezing and thawing + ammonium sulfate fractionation | Activated charcoal and chitosan + tangential cross-flow filtration | 4.3 | 8 | [87] |
PC | Phormidium fragile | Grinding under liquid nitrogen + ammonium sulfate fractionation | Hydrophobic interaction chromatography | 4.52 | [88] | |
PC | Arthronema | Freeze–thawed | Rivanol treatment | 4.52 | 55 | [89] |
APC | 2.41 | 35 | ||||
PC | Spirulina fusiformi | Freezing and thawing | Rivanol treatment | 4.3 | 46 | [90] |
PC | Aphanizomenon flos-aquae | Ammonium sulfate fractionation | Hydroxyapatite chromatography | 4.78 | [91] | |
PC | Spirulina (Arthrospira) platensis | Homogenized | Anion-exchange chromatography | 3.43 | 77.3 | [92] |
PC | Synechocystis aquatilis | Osmotic shock | Expanded bed chromatography + anion-exchange chromatography | > 4.0 | [93] | |
PC | Calothrix sp. | EDTA and lysozyme treatment | Anion chromatography + hydrophobic interaction chromatography | 3.5 | [94] | |
PC | Euhalothece sp. | Freezing and thawing + ammonium sulfate fractionation | Activated carbon adsorption + UF | 5 | 60 | [95] |
PC | Anabaena fertilissima UPCCC | Freezing and thawing + ammonium sulfate fractionation | Ion exchange chromatography + size exclusion chromatography | 3.28 | 28 | [96] |
PC | Synechococcu sp. | Ultrasound + ammonium sulfate fractionation | Ion-exchange (DEAE-cellulose) chromatography | 4.03 | [97] | |
PC | Spirulina (Arthrospira) platensis | Ultrasound + ammonium sulfate fractionation | Gel-filtration column | 3.5 | [98] | |
PC | Arthrospira platensis | Osmotic shock | Activated charcoal + Sephadex G 100 + DEAE sepharose fast Flow | 3.25 | 48.2 | [99] |
PC | Arthrospira platensis | Ultrasound | Activated carbon adsorption | 1.23 | 80 | [100] |
PC | Spirulina (Arthrospira) platensis | Milling + ammonium sulfate fractionation | Ion exchange chromatography | 4.0 | [101] | |
PC | Arthrospira platensis | Microwave | Ionic liquids | 1.22 | [102] | |
PE | 0.71 | |||||
APC | 1.03 | |||||
PC | Spirulina (Arthrospira) platensis | Freezing and thawing | ATPS | 2.38 | [103] | |
PC | Spirulina (Arthrospira) platensis | Freezing and thawing | ATPS + UF | 2.11 | [104] | |
C-PC | Spirulina (Arthrospira) platensis | Ultrasound | Liquid biphasic flotation technique | 3.49 | 90.4 | [105] |
PC | Arthrospira platensis | Ultrasound | ATPS + dialysis + precipitation | 4.22 | [106] |