Intron-mediated enhancement of DIACYLGLYCEROL ACYLTRANSFERASE1 expression in energycane promotes a step change for lipid accumulation in vegetative tissues

Table 1 Summary of TAG and TFA content and transgene expression in leaves of energycane

Construct	Lines	TAG (% of DW)	TFA (% of DW)	Transgene expressions normalized to GAPDH
Construct	Lines	TAG (% of DW)	TFA (% of DW)	WRI1	DGAT1	OLE1
None	WT	0.02 ± 0.01^a	1.38 ± 0.02^a	0.00 ± 0.00^a	0.00 ± 0.00^a	0.00 ± 0.00^a
WDO	L4	0.33 ± 0.05^bc	2.33 ± 0.05^bcd	0.00 ± 0.00^a	0.04 ± 0.01^ab	0.25 ± 0.10^abcd
WDO	L5	0.45 ± 0.02^bc	2.39 ± 0.05^bcd	0.01 ± 0.00^ab	0.06 ± 0.00^ab	0.09 ± 0.01^ab
WDO	L6	0.56 ± 0.02^bcd	2.78 ± 0.05^bcde	0.03 ± 0.01^c	0.09 ± 0.03^abc	0.19 ± 0.04^abc
WDO	L7	0.65 ± 0.01^bcd	2.96 ± 0.10^bcde	0.03 ± 0.00^c	0.10 ± 0.01^abc	0.31 ± 0.03^bcd
WDO	L8	1.01 ± 0.01^cde	3.05 ± 0.12^cde	0.05 ± 0.01^d	0.12 ± 0.01^abc	0.49 ± 0.07^d
WDO mean		0.60	2.70	0.02	0.08	0.27
WDiO	L9	0.23 ± 0.03^b	2.06 ± 0.08^ab	0.01 ± 0.01^ab	0.01 ± 0.00^a	0.02 ± 0.00^a
WDiO	L10	0.28 ± 0.01^bc	2.15 ± 0.11^abc	0.00 ± 0.00^a	0.06 ± 0.03^ab	0.21 ± 0.05^abc
WDiO	L11	0.93 ± 0.09^bcde	3.01 ± 0.05^bcde	0.02 ± 0.01^bc	0.79 ± 0.16^d	1.36 ± 0.13^e
WDiO	L12	2.68 ± 0.53^f	5.71 ± 0.64^f	0.03 ± 0.01^c	0.92 ± 0.10^d	1.22 ± 0.24^e
WDiO	L13	3.85 ± 0.65^g	8.39 ± 0.92^g	0.03 ± 0.01^c	1.01 ± 0.21^d	1.87 ± 0.14^f
WDiO mean		1.59	4.26	0.02	0.56	0.94

TAG values and gene expression are shown for each line representing leaf extracts from three replications. The leaf samples used are the first dewlap leaf. Values are means ± SE (n = 3). The expression of transgenes is shown relative to GAPDH. WT indicates the non-transgenic plants. WDO indicates the transgenic lines expressing WRI1, DGAT1 without intron, OLE1 and nptII. WDiO indicates the transgenic lines expressing WRI1, DGAT1 with intron, OLE1 and nptII. Bold values indicate the mean of all WDO or all WDiO lines, respectively. Values in the same column with different lower case letters are significantly different at p ≤ 0.05

ISSN: 2731-3654