Intron-mediated enhancement of DIACYLGLYCEROL ACYLTRANSFERASE1 expression in energycane promotes a step change for lipid accumulation in vegetative tissues

Table 3 Height, stem diameter and number of tillers of the transgenic energycane plants (L11; L12; L13) and non-modified energycane (WT) under greenhouse conditions

Construct	Line	Height (m)	No. of tillers	Stem diameter (cm)
None	WT	1.69 ± 0.16^a	16 ± 1.80^a	1.01 ± 0.11^a
WDiO	L11	1.45 ± 0.17^a	8 ± 0.66^b	0.85 ± 0.08^ab
WDiO	L12	0.99 ± 0.07^b	4 ± 0.81^c	0.62 ± 0.09^b
WDiO	L13	0.89 ± 0.09^b	3 ± 0.33^c	0.57 ± 0.08^b

TAG values and gene expression are shown for each line representing stem extracts from three replications. Values shown for each line represent the mean of mature, mid-mature and immature stem samples from three replications. Values are means ± SE (n = 3). The expression of transgenes is shown relative to GAPDH. WT indicates the non-transgenic plants. WDiO indicates the transgenic lines expressing WRI1, DGAT1 with intron, OLE1 and nptII

ISSN: 2731-3654